Posted: April 30th, 2022

Lab report about extracting DNA and amplifying

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write up a lab report about extracting DNA and amplifying and run the samples in yield gel write method and result and discussion and conclusion my samples are y1,y2,y3,y4,y5 extraction positive control, y6 extraction negative control, 676-p is pcr positive control and 676-n is pcr negative control the variable is the time y1 one day old cigarette, y2 3 days old, y3 6 days old, y4 is 9 days old
articipate in discussion about the results of your mock case samples.
Include a methods section – describe your samples. The extraction was with the DNA investigator kit and amping was done with the F15 and R389 primers and GoTaq PCR master mix. You can be very brief when describing those procedures (use the terms “manufacturers recommended protocol” and you do not have to give a step by step breakdown.
Include results. You can write these up in paragraph form. Include the yield gel photo as a figure and discuss why some bands may have appeared less intense than the others.
Include a conclusion paragraph about what, if anything, can be concluded.
Ask if you have questions.
I will post the mtDNA sequence alignment assignment as a separate assignment worth 0 points.
in the gel result picture my samples the four started in the edge not the middle.

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