Posted: October 24th, 2022

How will that modification improve the purification?

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How would you modify this purification strategy to maximize the yield and
purity of LDH? (40 points, 1 page/400-600 words) Which steps in the original
purification strategy worked well, and which did not? What would you change to get
better recovery and purification of LDH?
Give each step/technique of your modified strategy and describe what is accomplished
by that step. You can change the parameters or way that any individual technique is
done. You can also change the order of the techniques used, remove a technique, and
even add a new technique that you have not performed. Just be sure that you can
provide a rationale for each step in your new purification strategy based on the
purification table data. How will that modification improve the purification? You should
provide numbers from your purification table in making your arguments here.
Keep in mind that the initial crude homogenate is not listed in the purification table, and
the clarified homogenate is given as the starting material. This is because the crude
homogenate is very difficult to work with; its turbidity interferes with the assays and the
LDH is rapidly degraded by proteases. Even though you didn’t measure it, you may
assume the initial centrifugation you performed to isolate the cellular fraction containing
LDH achieved a good degree of purification from the crude homogenate. You should
account for this in your new purification strategy.
Put some careful thought and consideration into answering these questions. You score
on this is based on the quality of your writing, including how well you make your
arguments and provide a careful rationale for your answers.

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